摘要
通过对海洋真菌Fusariumsp.LD8产岩藻多糖酶发酵培养基组成及工艺条件的研究,得到较优培养基配方为:麸皮5%,海带粉2%,(NH4)2SO40.8%。以人工海水代替蒸馏水,接种量为3%,在恒温振荡器中以180r/min的速度振荡培养,岩藻多糖酶活可达2.10IU/mL。
The media components and cultivation conditions for fucoidanase production from Fusarium sp. LD8 were studied. The optimal fermentation nutrient components were as follow： wheat bran 5%, （NH4）2SO4 0.8% and laminaria powder 2%. The optimal inoculum was 3%. In the case, Fusarium sp. LD8 was cultured at 28℃ for 60 hours on a rotary shaker with a speed of 180 rpm, fucoidanase activity reached 2.10 IU/mL. The fucoidanase properties of LD8 were also studied.
出处
《菌物学报》
CAS
CSCD
北大核心
2006年第1期77-82,共6页
Mycosystema
基金
教育部留学回国人员科研启动基金
安徽省优秀青年科技基金资助(04043051)
安徽省自然科学基金资助项目(03043104)
安徽省教育厅中青年教师科研资助计划项目
合肥学院科学研究发展基金
关键词
培养基配方
振荡培养
接种量
酶学性质
fermentation nutrient component
optimal inoculum
shake culture
enzymatic properties
