摘要
目的:探讨仙慈丹对小鼠Lewis肺癌的生长抑制,免疫调节,细胞周期及凋亡的影响,并对其抗癌作用机制进行探讨。方法:选择C57BL/6小鼠移植性Lewis肺癌为实验模型进行试验,造模5 d后,分为模型对照组、顺铂给药组、仙慈丹高、中、低剂量组、联合用药组,给药13 d,给药剂量(mg.kg-1)分别为0,2.0(ip),0.648(ig),0.324(ig),0.162(ig),0.162(ig)+2.0(ip);观察仙慈丹有效部位对小鼠肺癌的抑制、免疫调节的影响,同时采用流式细胞仪检测肿瘤组织的细胞周期、凋亡率,应用免疫蛋白印记法(Western blot法)观察仙慈丹有效部位对p53,Bcl-2表达的影响。结果:①抑瘤率:阳性药物顺铂组、仙慈丹高、中、低各给药组、联合给药组分别为(63.02±3.21)%,(46.59±2.60)%,(42.48±2.77)%,(15.74±1.94)%,(64.56±1.62)%;②脾脏指数:仙慈丹高、中、低各给药组指数较高,与阳性药物组比较具有显著性差异(P<0.01);③荷瘤小鼠肿瘤细胞凋亡率:模型对照组、阳性药物组、仙慈丹高、中、低各给药组、联合给药组分别为0.04%,30.40%,14.80%,17.98%,16.04%,20.34%;④阳性药物组、仙慈丹高、中给药组、联合用药组的G0/G1细胞所占百分比与模型组比较具有显著性差异(P<0.01);⑤p53及Bcl-2表达量:仙慈丹高、中、低给药组及顺铂仙慈丹联合用药组表达量均降低,与模型组比较具有显著性差异(P<0.01)。结论:仙慈丹有效部位可以抑制Lewis肺癌生长,其抗癌作用与诱导细胞凋亡,调节细胞周期有关。
Objective： To investigate the inhibition effects of Xiancidan（XCD） on the lung-cancer cell in Lewis tumor-bearing mice and explore its mechanism. Method： C57BL/6 mice were taken as model, and divided into model group, cis-platinum group,high dose group XCD, middle dose group XCD, low dose group XCD, combination of XCD and control group 5 days later, All groups were administrated for 13 days,and the respective dosage was 0,2.0（ip）,0.648（ig）,0.324（ig）,0.162（ig）,0.162（ig）＋2.0（ip）.To observe the effect of XCD on inhibition of the lung cancer cell and immune regulation,AnnexinⅤ-FITC/PI method was applied to determine the apoptosis and cycle of transplanted cancer cells and Western blot method was used to observe the p53,Bcl-2 gene expression. Result： ①The inhibition rate in positive control group,high dose group, middle dose group, low dose groupof XCD, combination of XCD and control group were（63.02±3.21）%,（46.59±2.60）%,（42.48±2.77）%,（15.74±1.94）%,（64.56±1.62）%.②The pleen Index of high dose group XCD, middle dose group XCD and low dose group were significantly different from model group（P〈0.01）. ③The apoptosis rate of model group, control group,high dose group XCD, middle dose group XCD, low dose group XCD, the combination group of XCD and positive drug was 0.04%,30.40%,14.80%,17.98%,16.04%,20.34%, respectively.④Compared with model group, the percentage of G0/G1 cell in positive control group, high dose group XCD, middle dose group XCD and the combination groupm was significantly decreased. ⑤The p53 expression and Bcl-2 rate was significantly different from model group. Conclusion： The XCD effective parts can inhibit the growth of lewis tumor, which is related to its apoptosis-inducing and immune regulation effect.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期208-212,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科学技术厅社会发展项目(2010B030700001)
