摘要
目的:探讨六味地黄汤治疗原发性肝癌的作用机制。方法:将30只移植性原发性肝癌小鼠随机分为模型组、六味地黄汤组及5-FU组,每组10只。模型组和六味地黄汤组小鼠分别给予蒸馏水和六味地黄汤灌胃,每天1次,连续给药10天;5-FU组隔天给予5-FU腹腔注射。观察比较各组小鼠的体重、瘤重、血清血管内皮生长因子(VEGF)、肿瘤细胞凋亡及细胞周期。结果:小鼠体重和去瘤体重比较,六味地黄汤组显著高于5-FU组及模型组(P<0.01);血清VEGF值比较,六味地黄汤组低于模型组(P<0.01);肿瘤指数比较,六味地黄汤组显著低于模型组和5-FU组(P<0.01,P<0.05);各组S期细胞比较,六味地黄汤组显著低于模型组(P<0.01),高于5-FU组(P<0.01);各组G0-G1期细胞比较,六味地黄汤组高于模型组(P<0.01),与5-FU组之间差异不显著(P>0.05)。G2-M期细胞各组比较差异无统计学意义(P>0.05)。结论:六味地黄汤治疗原发性肝癌的作用机制可能是下调血清VEGF水平,影响瘤体细胞周期,诱导肝癌细胞凋亡。
Objective： To investigate the mechanism by which Liuwei Dihuang decoction exerts therapeutic effect on primary liver cancer. Methods： Thirty mice with transplanted primary liver cancer were randomly divided into model group （n = 10）, Liuwei Dihuang decoction group （n = 10）, and 5 -fluorouracil （5 -FU） treatment group （n = 10）. The model group and Liuwei Dihuang decoction group were given distilled water （once daily） and Liuwei Dihuang decoction （once daily）, respectively, for 10 days; the 5 -FU treat- ment group was intraperitoneally injected with 5 - FU. These groups were compared in terms of body weight, tumor weight, serum vascu- lar endothelial growth factor （VEGF） level, tumor cell apoptosis, and cell cycle. Results： The body weight and tumor- free body weight were significantly higher in the Liuwei Dihuang decoction group than in the 5 - FU treatment group and model group （ P 〈 0.01 ）. The Li- uwei Dihuang decoction group had a significantly lower serum VEGF level than the model group （ P 〈 0.01 ） and a significantly lower tumor index than the model group and 5 - FU treatment group （ P 〈 0.01 or P 〈 0.05 ）. Compared with the Liuwei Dihuang decoction group, the model group had a significantly higher percentage of cells in S phase （P 〈 0.01 ） ; the 5 -FU treatment group had a signifi- candy lower percentage of cells in S phase （ P 〈0.01 ） ; the model group had a significantly lower percentage of ceils in both GO and G1 phases （P 〈 0.01 ）. There was no significant difference in the percentage of cells in both GO and G1 phases between the Liuwei Dihuang decoction group and 5 - FU treatment group （ P 〉 0.05 ）. Also, there were no significant differences in the percentage of cells in both G2 and M phases among the three groups （ P 〉 0. 05 ）. Conclusion ： Liuwei Dihuang decoction exerts therapeutic effect on primary liver canc- er probably by downregnlating serum VEGF level, affecting tumor cell cycle, and inducing liver cancer cell apoptosis.
出处
《湖南中医杂志》
2013年第2期124-126,共3页
Hunan Journal of Traditional Chinese Medicine
关键词
原发性肝癌
六味地黄汤
血管内皮生长因子
细胞凋亡
小鼠
实验研究
primary liver cancer
Liuwei Dihuang decoction
vascular endothelial growth factor
cell apoptosis
mouse
experi-mental study
