摘要
CD1d限制性NKT细胞(自然杀伤T细胞)是一群具有免疫调节功能的T细胞亚群,在调控多种免疫应答中发挥重要作用.MicroRNAs介导的RNA干扰已被证明是调控NKT细胞发育和功能的关键分子机制,然而特异microRNA在NKT细胞发育和功能中的作用目前仍不清楚.在本研究中,我们采用miR-150基因敲除小鼠以及流式细胞术、ELISA等方法,观察miR-150对小鼠NKT细胞发育和功能的影响.结果表明:miR-150基因缺失致小鼠胸腺NKT细胞数量减少,但不影响外周NKT细胞的数量;活化后miR-150敲除小鼠NKT细胞与野生型小鼠NKT细胞相比,IFN-γ产生增加.进一步采用小鼠黑色素瘤模型观察miR-150对肿瘤细胞转移的影响,发现miR-150敲除显著增强半乳糖神经鞘氨醇(α-Galcer)对小鼠黑色素瘤细胞肺转移的抑制效果.上述结果为特异microRNA调控NKT细胞发育和功能的研究提供了新的线索.
CDld-restricted natural killer T cell （NKT） is a subset of T cells and plays an important role in the regulation of diverse immune responses. MicroRNA-mediated RNA interference is emerging as a crucial regulatory mechanism in the control of NKT cell development and function. Yet, roles of specific microRNA in the development and function of NKT cells is not completely understood. In this study, miR-150 knockout （miR-150KO） mice were adopted and the quantities of thymic and peripheral NKT cells were detected by flow cytometry. Cytokine production was detected by intracellular staining and ELISA. We found that miR-150 deletion resulted in the decreased number of thymic NKT cells, while peripheral NKT cells did not change in mice. However, activated NKT cells in miR-150KO mice produced more IFN-γ than that of wild type control （WT） mice. In addition, using B 16BL6 melanoma mouse model, we found that miR-150 deletion enhanced the inhibitory effect of α-Galcer on the lung metastasis of melanoma ceils. Our data provide new clues for the specific role of miR-150 in the development and function of NKT ceils.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2013年第5期454-460,共7页
Progress In Biochemistry and Biophysics
基金
河北省唐山市科技局指令性科研项目(12140209A-44)~~
