摘要
本文应用人胃癌组织及细胞株移植建立了裸大鼠和裸小鼠两种胄癌移植模型。用^(125)I和^(131)I标记抗人胃癌单克隆抗体1D1-2,体外试验表明碘化1D1-2具有特异性的结合活性。注入荷瘤裸鼠体内72h后,显示该抗体高度浓集于肿瘤组织,浓聚指数为4.5,而正常组织仅为1.3,T/NT比分别为2～12倍,与胃组织比为3.2倍、放射免疫显像显示出清晰的肿瘤图像。提示单克隆抗体1D1-2应用于肿瘤定位诊断及导向治疗的可能性。
Two kinds of models of rude mice and rats bearing the human gastric cancer were established from fresh surgical specimens and cell line (GGC-8340), Tumor lustological structure and cell morphology were similar before and after transplantation. The monoclonal antibody GMG1:1 D 1-2 against human gastric cancer was labeled by 125I and 131I. The binding radioactivity of radio -iodinated 1D1-2 with GGC-8140 was determined by vitro binding assay, suggesting ihat 1D1-2 reacted specifically with GGC-8140. Radioimmunodetection study showed that 72 hr after injection of 125I-1D1-2, all the two kinds of xenograf;s were specifically located by 1 D 1-2. Tumor location index was 4.5, while that of the normal tissue was only 1. 3. The ratio of tumor to normal tissue ( T/NT ) in the brain was 12; T/NT in the slomach was 3.2; T/NT in the blood increased gradually and was 2, 0. Radioimmunoimaging was performed in tumor bearing nude mice and rats. The tumor was clearly visualized 72 hr after injection of 125I-1D1-2. Radioactivity was higher in tumor region than in other region. It is suggested that McAb:1D1-2 has the possibility of location in the tumor diagnosis and the treatment guidance.
关键词
放射免疫显像
胃肿瘤
单克隆抗体
antibody, monoclonal
tumor-bearing nude mice
radioimmunoimaging
