摘要
为优化猫棒束孢真菌多糖提取工艺,采用液体发酵法培养猫棒束孢菌丝体,采用水溶醇沉法提取猫棒束孢真菌多糖,采用苯酚-硫酸比色法测定多糖含量,通过单因素实验和L9(33)正交实验,确定猫棒束孢真菌多糖的最佳提取工艺条件为:水料比6:1,提取液浓缩至原料重量的2倍,加入浓缩液4倍的无水乙醇,在此条件下多糖的提取率为16.24%。
Isaria felina was a new strain of Cordyceps sinensis that had been separated from the fructification of natural Cordyceps sinensis. The purpose of this article was to study the optimum extraction technology of polysaccharide from Isaria felina.The method of liquid fermentation was used to culture Isaria felina, the method of water extraction and alcohol precipitate was used to isolate polysaccharide from the mycelium of Isaria felina,and the method of phenol-sulfuric acid was used to determine the content of polysaccharide.Through the single factor and orthogonal test, result showed that the optimum extraction conditions were as follows ： the ratio of water and mycelium powder was 6：1 ,the extract was concentrated to twice the weight of mycelium powder and the ethanol ratio was four times the volume of the concentrated extract. Under this condition, the extraction rate of polysaccharides was 16.24%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第11期248-250,共3页
Science and Technology of Food Industry
基金
山西省实验动物专项资金项目(2012K05)
关键词
猫棒束孢
多糖
提取工艺
Isaria felina
polysaccharide
extraction technology
