摘要
目的制定生发乌发口服液的质量标准。方法采用薄层色谱法(TLC)对处方中的制何首乌和黄芪进行定性鉴别,采用高效液相色谱法(HPLC)对制剂中的二苯乙烯苷含量进行定量测定。结果 TLC法能定性检出制何首乌和黄芪,斑点清晰,且阴性对照无干扰。HPLC法测定二苯乙烯苷在1.6～80.0μg.mL-1范围内线性关系良好,r=0.999 9;平均加样回收率为99.2%,RSD为2.0%(n=6)。结论该研究所建立的定性、定量检测方法操作简便,结果准确可靠,重复性好,能有效地控制生发乌发口服液的质量。
Objective To establish the quality standard for Shengfa Wufa Oral Solution. Method The qualitative identification of Poly- goni Multiflori Radix Praeparata and Astragali Radix was carried out by TLC, and the content of stilbene glycoside was determined by HPLC. Results The results of TLC showed that the relevant spots were clear,and no interference was observed. The content of stilbene glycoside showed a good linearity in the range of 1.6-80.0 μg · mL-1 ,r=0. 999 9. The average recovery rate was 99.2% and RSD was 2.0 % （n= 6）. Conclusion The established qualitative and quantitative methods are simple and accurate, reliable and reproducible,and can be effectively used for the quality control of Shengfa Wufa Oral Solution.
出处
《西北药学杂志》
CAS
2013年第3期235-237,共3页
Northwest Pharmaceutical Journal
基金
广西中医学院自然科学研究课题(编号:P2010065)
广西中医学院第一附属医院青年科研基金课题(编号:GZYQJ2011025)
