摘要
目的观察外源野生型 p5 3基因在肝癌基因治疗方面的可行性。方法将载有人野生型 p5 3- c DNA的真核表达质粒 p5 3- pc DNA3,用阳离子脂质体介导转染人肝癌细胞系 Hep G2 ,用流式细胞仪检测 p5 3- pc DNA3对 Hep G2细胞生长的影响。结果通过观察细胞生长曲线与流式细胞仪检测细胞周期和细胞的凋亡指数发现 ,Hep G2细胞生长受到明显的抑制。结论脂质体介导的 p5 3基因可在 Hep G2细胞中表达 。
ObjectiveTo study the feasibility of the use of exogenous wild type p53 in gene therapy for liver cancer.MethodsRecombinant eukaryotic expressing plasmid p53 pcDNA 3 containing human wild type p53 cDNA was transiently introduced into human liver cancer cell line HepG2 mediated by cation iron lipofectamin and selected by G418.By flowcytometric analysis,the effects of p53 pcDNA 3 on the growth of liver cancer cells were evaluated.ResultsThe cell growth curve,cell cycle, apoptosis index of HepG2(control cells) were compared with those of HepG2 p53 cells.The growth of HepG2 p53 cells was greatly suppressed.ConclusionExogenous wild type p53 gene may stably exist in HepG2 cell after being mediated with lipofectamin.These results indicate that recombinant plasmid expressing wild type p53 may be useful for gene therapy of human liver cancer.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2000年第5期359-361,369,共4页
Immunological Journal
基金
天津市卫生局科研课题资助项目!(98KYGG-1)
