摘要
观察外源野生型p5 3基因在肝癌基因治疗方面的可行性。方法 :将载有人野生型 p5 3-cDNA的真核表达质粒pcDNA3 - p5 3,用阳离子脂质体介导转染人肝癌细胞系HepG3B细胞 ,用流式细胞仪检测pcDNA3-p5 3对HepG3B细胞生长的影响。结果 :通过观察细胞生长曲线与流式细胞仪检测细胞周期和细胞的凋亡指数发现 ,HepG3B细胞生长受到明显的抑制。结论 :脂质体介导的p5 3基因可在HepG3B细胞中表达 ,且明显抑制该细胞的生长。
Objective:To study the feasibility of the use of exogenous wild type p53 in gene therapy for liver cancer.Methods:Recombinant eukaryotic expressing plasmid pcDNA 3-p53 containing human wild-type p53-cDNA was transiently introduced into human liver cancer cell line HepG3B mediated by cation iron lipofectamin and selected by G418.By flowcytometric analysis,the effects of pcDNA 3-p53 on the growth of liver cancer cells were evaluated.Results:The cell growth curve,cell cycle,apoptosis index of HepG3B(control cells) were compared with those of HepG3B-p53 cells.The growth of HepG3B-p53 cells was greatly suppressed.Conclusion:Exogenous wild type p53 gene may stably exist in HepG3B cell after being mediated with lipofectamin.These results indicate that recombinant plasmid expressing wild type p53 may be useful for gene therapy of human liver cancer.
出处
《中国现代医学杂志》
CAS
CSCD
2002年第15期5-7,共3页
China Journal of Modern Medicine
