摘要
应用同源重组方法成功地构建了携带野生型ｐ５３基因的复制缺陷型重组腺病毒，通过光镜、电镜、酶切、ＰＣＲ共扩增等方法证实了构建载体的正确性，并使常规的同源重组方法进一步改进，简化了载体构建的操作程序，结果准确，成功率高。本结果可用于以腺病毒为载体进行肿瘤、心血管疾病等基因治疗的研究。
A replication defective recombinant adenoviruses that coding wild type p53 gene were constructed by means of homologous recombination.The structure of the recombinant adenovirus were confirmed by light and electronic microscopy,restriction analysis and PCR co amplication.Our procedure simplified the routine protocol of homologous recombinant with improved result.This work could be used in the therapy of tumor and cardiovascular diseases which mediated by recombinant adenoviruses.
出处
《哈尔滨医科大学学报》
CAS
1997年第6期441-443,共3页
Journal of Harbin Medical University
关键词
腺病毒
载体
同源重组
P53基因
基因疗法
Gene therapy
Adenovirus vectors
Homologous recombinant
p53 gene
