摘要
目的通过体外分离、增殖培养获取 γδΤ细胞、NK细胞和 L AK细胞 ,并比较 3种细胞的抗肿瘤的生物学特性。方法收集用不同单抗分别包被粘附的细胞 ,通过 MACS细胞分选仪的分选 ,获取的细胞进行细胞增殖动力学、细胞表型、细胞杀伤活性的测定以及单抗阻断效应的分析。结果经 MACS分离得到的 γδΤ细胞 ,培养 2周后细胞数扩增 6 0 0～ 80 0倍 ,CD3、CD8和 γδ细胞表达阳性率分别是 72 .2 9%、5 8.0 2 %和 6 5 .98%。γδΤ细胞对 NK敏感细胞 K5 6 2以及 NK不敏感细胞 Raji和 XG- 7这 3种不同靶细胞均有较高的杀伤率 ,分别为 35 .98%、5 2 .2 7%和 6 9.0 8;NK细胞对此 3种细胞的杀伤率分别是45 .2 1%、12 .34%和 2 9.2 7% ;L AK细胞的杀伤率分别为 44 .0 1%、2 9.2 7%和 2 5 .6 8%。γδΤ细胞对经 MHC- 类单抗阻断前后的 K5 6 2、Raji和 XG- 73种靶细胞的杀伤率无明显改变。结论γδΤ细胞、NK细胞和 L AK细胞都具有一定非特异性杀伤肿瘤细胞的作用 ;γδΤ细胞对 MHC- 类单抗阻断后的肿瘤细胞的杀伤无明显变化。提示 γδΤ细胞较 NK细胞和 L
ObjectiveAs the function of γδ T cells and NK in the immunological therapy becomes more and more important,the characteristics of γδ T cells,NK and LAK were analyzed comparatively after that they were amplified by isolating and incubating in vitro . MethodsThe cells were collected using MACS after the cells were panned respectively with special monoclonal antibodies.Then the characteristics including proliferation,phenotype,cytotoxin and the down regulation blocked by specific antibodies were analyzed.Results The γδ T cells can expand 600～800 times after incubation for 2 weeks,and the percent of CD3,CD8 and γδ expressed on the collected cells were 72.29%,58.02% and 65.98% respectively.γδ T cells showed high cytotoxcity to K562 (NK sensitive tumor cell line),Raji and XG 7 cell lines (NK non sensitive tumor cell lines).The percentage of cytotoxin reached 35.98%,52.27% and 69.08% respectively compared with γδ percent of cytotoxin of PBMC only revealed 19.56%,11.34% and 10 58%,of NK showed 45.21%,12.34% and 29.27% while that of LAK were 44.01%,29.27% and 25.68% respectively.No obvious change of percent of γδ cytotoxic ability to these target cells were observed using special MHC class Ⅰ monoclonal antibody to block the γδ cell before coculturing the target cells with γδ T cells.Conclusion All of γδ T cells,NK and LAK showed the non specific cytotoxin to tumor cells.The cytotoxic capability of γδ T cells wad not affected after blocking with MHC class Ⅰ monoclonal antibody.These results demonstrated that γδ T cell could kill more kinds of tumor cells than NK and LAK.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2000年第5期346-348,共3页
Immunological Journal
