摘要
以艾蒿为原料,在确定最佳提取溶剂基础上提取艾蒿中的抗菌成分,利用中心组合(Box-Behnken)试验设计原理,采用3因素3水平响应面分析法,获得多元二次线性回归方程,以艾蒿提取物抑菌圈直径为响应值做响应面和等高线图,对主要工艺参数进行优化并得到回归模型。结果表明:以乙醇为提取溶剂,提取艾蒿中抗菌成分的最佳工艺条件为:乙醇浓度62%、浸提温度72℃、浸提时间8h、料液比1:20,该条件下的艾蒿抗菌成分提取液对大肠杆菌的抑菌圈直径为2.47cm。
Takes Artemisia argyi as the primary raw material, the optimal solvent was first determined to extract antibacterial ingredient in Artemisia argyi, a multiple quadratic regression describing the relationship between antibacterial circle diameter and extraction parameters such as ethanol concentration, extraction temperature and extraction time was established using a 3-factor, 3-level Box- Behnken statistical design, and response surface and contour plots with the extraction antibacterial ingredient in Artemisia argyi as the response were drawn based on the model. The optimal levels for each variable to obtain the highest diameter of inhibition zones were as follows： ethanol concentration 62%, extraction temperature 72 ℃, extraction time 8 h, and ratio of liquid material 1：20. Under such conditions, selecting ethanol as solvent, the diameter of inhibition zone reached 2.47 cm.
出处
《食品科技》
CAS
北大核心
2013年第5期244-249,共6页
Food Science and Technology
基金
吉林省重大科技项目(20060208)
关键词
艾蒿
抗菌成分
提取
响应面法
Artemisia argyi
antibacterial ingredients
extract
response surface analysis methodology（RSM）
