摘要
本研究采用超声波辅助乙醇提取法提取野生艾蒿中抑菌成分,琼脂空穴扩散法检测艾蒿提取物抑制大肠杆菌能力。通过检测抑菌圈直径大小得出最佳提取工艺参数。结果表明,提取最佳工艺条件为:超声功率270W、超声时间35min、乙醇浓度51%、浸提温度73℃、乙醇浸提时间6h、料液比1∶15,该条件下,获得的艾蒿抗菌成分抑菌圈直径为2.62cm,与回归模型数据分析结果的理论预测值2.78cm相比相对误差为6.5%。
Ultrasonic extraction assisted with ethanol was used to extract the antibacterial components of wild Artemisia,and then agar- hole- diffusion method was applied to detect its inhibition ability to E.colLThe optimal extraction parameters could be obtained by measuring bacteriostatic circle diameter.The results showed that the optimum extraction conditions were., ultrasonic power 270W, ultrasonic time 35min, ethanol concentration 51%, extraction temperature 73℃, ethanol extraction time 6h, ratio of solid to liquid 1：15.With this condition, the E col/ inhibition zone diameter of wild artemisia antibacterial component was 2.62cm, comparing with the theoretical
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第23期247-252,共6页
Science and Technology of Food Industry
关键词
艾蒿
超声波辅助
抗菌成分
Artemisia argyi
ultrasonic extraction
antibacterial ingredients
