摘要
目的探讨Smad4的表达与结直肠癌肝转移的关系。方法通过免疫组化方法检测79例结直肠癌组织和20例癌旁正常组织中Smad4蛋白的表达,选择性别、肿瘤部位、肿瘤大小、肿瘤分化程度、淋巴结转移、大体分型、术前血清CEA水平和Smad4蛋白表达等8个可能影响肝转移的因素,用χ2检验和logistic回归对肝转移的影响因素进行单因素和多因素分析。结果 Smad4蛋白在结直肠癌组织中表达减少,阴性表达率为72.2%(57/79),在结直肠正常组织中阴性表达率为5.0%(1/20),差异有统计学意义(P<0.01)。单因素分析显示,肿瘤分化程度、淋巴结转移、术前血清CEA和Smad4蛋白表达与肝转移有关(P<0.05);多因素分析显示,淋巴结转移、术前血清CEA水平和Smad4蛋白表达与肝转移相关,是结直肠癌肝转移的独立影响因素(OR值分别为6.062、3.725、0.170,均P<0.05)。结论 Smad4蛋白的表达缺失与结直肠癌肝转移明显相关,可作为一个新的预测结直肠癌肝转移的生物指标。淋巴结转移、术前血清CEA水平和Smad4蛋白表达是结直肠癌肝转移的独立影响因素。
Objective To investigate the correlation between the expression of Smad4 and liver metastasis ot colorectal carcinoma. Methods The expression of Smad4 in 79 colorectal carcinoma samples and 20 normal coloreetal samples were assessed by SP immunohistochemistry. Sex, localization of tumor, tumor size, differentiation grade, lymph node metastasis, gross tumor type, preoperative serum CEA, expression of Smad4 were enrolled for Z2 test and logistic regression analysis. Results The negative expression of Smad4 in colorectal carcinoma tissue was 72. 2% （ 57/79 ）, while 5.0% （1/20） in normal colorectal tissue, with significant difference between them （P 〈 0.01 ）. Differentiation grade, lymph node metastasis, preoperative serum CEA and expression of Smad4 were significantly correlated with liver metastasis according to single factor analysts （ P 〈0.05）. However, only lympk Bode metastasis, preoperative serum CEA and expression of Smad4 were significantly correlated with liver metastasis according to muhifactor analysis （ OR = 6. 062, 3.725, and 0. 170 ; P 〈 0.05）. Conclusion The down - regulation of Smad4 is correlated with liver metastasis of coloreetal carcinoma, providing a novel biomarker to predict liver metastasis of eolorectal carcinoma. Lymph node metastasis, preoperative serum CEA and expression of Smad4 are independent risk factors of liver metastasis of colorectal carcinoma.
出处
《广东医学》
CAS
CSCD
北大核心
2013年第8期1182-1185,共4页
Guangdong Medical Journal
