摘要
利用实时定量PCR方法,检测大豆class Ⅲ酸性内切几丁质酶基因在大豆各组织中的表达,结果显示该基因在大豆根、茎、叶中的表达活性低,而花和种子中的相对表达活性极高。利用PCR方法,克隆大豆class Ⅲ酸性内切几丁质酶基因5’端上游2 000bp序列,命名为CP。在线启动子预测软件分析,结果表明CP序列中含有多种典型的种子特异表达元件和花特异表达的元件,如SEF4 motif、E-box、G-box、(CA)n、AACA、ACGT、CCAA;52-box、ntp303-box、GTGA、TACPyAT box。推测大豆class Ⅲ酸性内切几丁质酶基因启动子具有调控下游基因在花和种子中大量表达的特性。
The expression pattern of soybean class Ⅲ acidic endochitinase gene in different soybean tissues was detected by real-time quantitative PCR （RTQ-PCR）. Results showed this enzyme activity was lower in roots, stems and leaves, but higher in flowers and seeds. 5’-flanking upstream 2000bp sequence of soybean class Ⅲ acidic endochitinase gene was isolated from soybean genomic DNA by PCR, and named CP. Sequence analysis revealed that this fragment contained a series of motifs related to seed-specific promoters and some pollen-expressed elements, such as SEF4 motif, E-box, G-box, （CA）n, AACA, ACGT, CCAA, 52-box, ntp303-box, GTGA, TACPyATbox. It was inferred that CP promoter could drive downstream gene over-expression in soybean flowers and seeds.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2013年第2期221-224,共4页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31070180)
黑龙江省教育厅科学技术研究项目(12521611)
作物生物学国家重点实验室开放基金(2012KF13)
齐齐哈尔大学青年教师科研启动支持计划(2010K-M09)
