摘要
本研究以几丁质酶基因导入玉米自交系海92-1所获得的转基因植株的种子作为试验材料,对其T0代种子进行了潮霉素抗性筛选试验,结果表明T1代的分离比分别为3:1和15:1(表1)。这证实大多数转基因植株中几丁质酶基因是以单拷贝、单位点整合到植株的基因组中,个别转基因植株中是以单拷贝、多位点整合到转基因植株的基因组中的。农艺性状调查分析,转基因株系的株高和穗位高都高于对照植株,单株结穗数基本不变,而转基因植株的穗长值和穗粒数值均比对照植株大,穗长增加4～7cm,穗粒数增加1%～7%。对T1,T2,T3代株系的PCR检测结果说明目的基因在转基因植株中可稳定遗传。田间抗病鉴定结果显示,转基因株系的发病率比当代对照株系明显减少2～4级,选育得到的纯合系06006和06012的发病率为0,表现出高抗病性。
In this study, the transgenic seeds used as experiment material were obtained from head smut-resistant transgenic maize lines that transformed by pollen- mediated approach on inbred line Hai 92-1. Partly T0 seeds were subjected to hygromycin- screening, the results appeared that the segregation ratio of majority of T1 transgenic lines for hygromycin-resistant and susceptible was 3：1, minority of T1 lines were 15：1. Further analysis suggested that single copy of the chitinase gene was integrated into the genome of most To transgenic plants, and a few of transgenic plants contained more than one copy of the gene. Investigation results oftransgenic plants on agronomic characters showed： firstly the plants height of transgenic lines were higher than that of control plants; secondly the era number per transgenic plant was as much as that of control; and next the ear length and Kernels / ear of transgenic lines were increase much than that of control plants, the degree of increase were 4-7 cm and 1%-7%, respectively. The PCR analysis for T1, T2, and T3 progenies indicated that chitinase gene can stablely inherited. Investigation results of disease incidence of transgenic lines included： the disease incidence of transgenic lines was 2-4 degree lower than CK lines. The disease incidence of lines 06006 and 06012 obtained from T3 generation transgeneic line by selection breeding.
出处
《分子植物育种》
CAS
CSCD
2007年第4期467-474,共8页
Molecular Plant Breeding
基金
国家863项目(2001AA212131)资助。
关键词
玉米
丝黑穗病
转基因
选育
Maize, Head smut, Transgene, Selection breeding
