摘要
以产油酵母菌Y1为试验菌株,通过对出发菌株Y1的最佳微波诱变时间确定、诱变后菌株初筛和复筛后得到一株突变菌株Y13,测得Y13的油脂产量4.39 g/L、油脂含量22.09%,比出发菌株Y1油脂产量2.11 g/L、含油量9.6%明显提高。因此,菌株Y13可作为后续摇瓶发酵产脂研究的试验菌株。
The mutant strain Y13 was isolated by the time of optimum microwave mutagenesis, mutagenic strains of screening and re-screening within oleaginous yeast Y1. The results showed that Y13 was significantly improved in oil production and content, the oil production reached 4.39 g/L from 2.11 g/L and the content reached 22.09% from 9.6%, respectively. It is feasible to employ Y13 as original strains using shake-flask fermentation for lipid-producing.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第6期118-119,123,共3页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31160254)
校内青年科学研究基金(BSYKJ2011-17)
关键词
产油酵母
微波诱变
诱变选育
油脂产量
oleaginous yeast
microwave mutation
mutation breeding
oil production
