摘要
从云杉树枝中筛选出1株高产油脂酵母菌Y-3,通过单因素试验对该菌发酵培养基的碳源、氮源、无机盐和培养条件(接种量、摇床转速、pH值、温度、时间等)进行了研究。结果表明,用酵母菌Y-3生产油脂的最适碳源和氮源分别为葡萄糖和硫酸铵,最适培养条件为:接种量为20%,初始pH值为6,培养温度为28℃,转速为160r/min,培养时间为6d。
A yeast named Y-3 with high oil-producing capacity was screened from spruce. The research was done on the respect of C-source, N-source and cultivation conditions by single factors experiment using the biomass, the content of microbial oils. The optimal fermentation conditions were decided as follows： the optimal carbon and nitrogen source for Y-3 to produce microbial oil were glucose and （NH4）2SO4, the optimal culture conditions at that the amount of inoculation was 20%, initial pH was 6.0, fermentation temperature was 28 ℃, rotational speed was 160 r/min and culture time was 6 d.
出处
《农产品加工（下）》
2010年第7期66-69,共4页
Farm Products Processing
关键词
产油酵母
生物量
油脂含量
发酵条件优化
oleaginous yeasts
biomass
content of microbial oils
optimization of fermentation conditions
