摘要
采用Real time PCR(RT-PCR)对患者鼻咽拭子样品进行检测,筛选新甲型H1N1流感病毒。将阳性样品接种MDCK细胞进行病毒分离,通过RT-PCR扩增病毒HA基因并进行测序分析,分析扬州市2009～2011年新甲型H1N1流感病毒血凝素(HA)基因的遗传进化特征。结果表明:分离出9株新甲型H1N1流感病毒,其HA基因核苷酸及氨基酸同源性分别为98.1%～99.8%和96.6%～99.8%;与疫苗株A/California/07/2009相比,核苷酸及氨基酸的同源性分别为98.4%～99.5%和97.0%～99.3%,其中2011年3株病毒在抗原位点上发生了点突变。遗传进化分析显示9株病毒形成3个明显的分支。证明新甲型H1N1流感病毒已发生了一定抗原漂移。
Respiratory tract swabs were collected and screened by a Real-time PCR method using a new influenza A （H1N1） virus detection kit. The positive samples were inoculated into MDCK cells for virus isolation. The HA genes of influenza A viruses were amplified by RT-PCR and were subject to sequence. The genetic characteristic of HA genes of new influenza A （H1N1） viruses isolated in Yangzhou area between 2009 and 2011 was analysed. The results showed that 9 new influenza A （HIN1） viruses were isolated. Their nucleotide and amino acid homologies of HA genes among 9 iso- lates were 98.1%- 99.8 % and 96.6 %- 99.8 %, respectively. Compared with vaccine strain A/California/07/2009, the nucleotide and amino acid homologies of HA genes were 98.4%--99.5% and 97.0%--99.3%, respectively. There were point mutations in antigenic epitope of HA protein of three viruses isolated in 2011. Polygenetic analysis revealed that there were three sublineage derived from 9 isolates. These data indicate that antigenic drift of HA protein has occurred among these new influenza A （H1N1） virus isolates.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2013年第1期36-40,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家重点基础研究发展计划项目(973-2011CB505003)
