摘要
为建立快速、灵敏的牛病毒性腹泻病毒（BVDV）的检测方法，根据GenBank已发表的序列，在其5’端非结构蛋白区域设计了1对特异性引物，用来检测BVDV，PEDV，SRV，TGEV等，同时建立了牛病毒性腹泻纳米PCR检测方法，经优化找出最佳的NanoPCR反应条件。经过检测，纳米PCR的最小检出量为15．2×10^-4，PCR的最小检出量为15．2×10^-3；BVDV的敏感性试验表明纳米PCR方法的敏感性是普通PCR的10倍。该BVDV纳米PCR具有较高的敏感性和特异性，是一种高效的检测手段，可应用于流行病学调查，同时也为BVDV的及时检测、预防和治疗奠定基础。
In order to develpop a rapid and sensitive assay for detection of BVDV,a pair of amplified 222 bp fragment specific primers to detect BVDV, PEDV, SRV, TGEV, etc based on the 5＇-un- translated region gene of bovine viral diarrhea virus（BVDV）. At the same time,developing a nano- particle assisted PCR assay for BVDV and finding the best reaction condition of a nanoparticle-as- sisted PCR. According the result, the minimum detectable amount of nanoparticle-assisted PCR was 15.2 × 10^- 4 ,the minimum detectable amount of PCR was 15.2 × 10^-3 ,the sensitivity of the nanoparticle-assisted PCR was 10 times more than the normal PCR. The BVDV nanoparticle-assis- ted PCR with high sensitivity and specificity,is a highly effective means of detection,it can be ap plied to epidemiological survey, but also lay a foundation for the timely detection, prevention and treatment of BVDV.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第2期252-254,共3页
Chinese Journal of Veterinary Science
