摘要
目的研究中国湖南地区人偏肺病毒（hMPV）的基因分型及其膜蛋白G序列的遗传学特征，比较分析其区域性流行特点。方法收集2005年中国湖南地区232份住院儿童鼻咽抽吸物（NPA）样本进行常见呼吸道病毒筛查，并扩增hMPV阳性样本的膜蛋白G全序列，与GenBank中已知hMPV参考株及其他地区公布的相关资料进行比对、进化等分子遗传学特征分析。结果从232份临床样本中共检测得到17份（7．3％）hMPV阳性样本，与其他病毒混合感染率达35％。扩增出其中13份hMPV样本的G蛋白全序列，分属于4种亚型（A1、A2、B1、B2）。核酸长度类型有4种（711，675，660，696nt），N-连接糖基化位点数目和位置、半胱氨酸残基数目等特征与已报道的同期北京地区、日本、北美等地区hMPV调查分析结果不尽一致。结论中国湖南地区与其他地区同期hMPV调查分析结果各有特点，反映出hMPV变异显著，具有明显的地区流行特征。
Objective To understand the genotypes of human metapneumovirus （hMPV） and the genetic character of hMPV attachment protein G sequence in Hunan, China. Methods 232 nasopharyngeal aspirates （NPA） samples from hospitalized children with acute respiratory infections were collected from Hunan, China in 2005. HMPV was detected. The full length of G glycoprotein genes were amplified and sequenced. Bioinformatics soft-wares were employed to analyze the sequences. Results 17/232 （7.3 % ） were showed hMPV positive. And co-infection rate with other viruses is 35%. The diagnoses of these hMPV positive cases are pneumonia, bronehiolitis and bronehopneumonia. Phylogenetic analysis for G genes from 13 hMPVs revealed the existence of four major subgroups： AI, A2, BI, B2 in Hunan, China in 2005. There are four types of sequence lengths of hMPV G glyeoprotein, which are 711, 675, 660, 696nt. It is different in potential N-linked glycosylation sites and number of eysteine residues among these hMPVs of Hunan, China and Beijing, China. Also it is different from those in Japan and North America. Conclusion The investigation of hMPV from Hunan, China in 2005 revealed the high speed of genetic variation and the marked character of geographic epidemic differences.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第2期101-103,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
呼吸道感染
变性肺病毒
病毒包膜蛋白质类
序列分析
Respiratory tract infections
Metapneumovirus
Viral envelope proteins
Sequence analysis
