摘要
采用3-氨基丙基-三甲氧基硅烷((3-aminopropyl)trimethoxysilane,APTES)和戊二醛(glutaraldehyde,GA)、左旋多巴(L-DOPA)修饰芯片载体表面,对两种不同修饰方法制备的蛋白质芯片进行对比研究。以XPS检测表面活化剂的修饰效果;将AFP抗体固定在修饰后的片基上,加入AFP溶液,以蛋白探针的反应性作为监测指标,通过LPAS系统检测反应产生的光电流。结果显示,左旋多巴修饰硅片对蛋白固定效果较好,有较高的反应活性,检测范围较宽,但幅值较小。
The surface of chips were modified by 3 - aminopropyl - trimethoxysilane （ （ 3-aminopropyl ） trimethyoxysilane APTES ） and glutaraldehyde （ Glutaraldehyde, GA ） , levodopa （ L-DOPA ） modification of the chip carrier surface. The two kinds of different types of modification of the protein chips prepared a comparative study. XPS detect surface modification effect of the activator. AFP antibodies were fixed on the modified film base, then adding AFP solution, and photocurrent detected by LPAS system, selecting reactive protein probes as monitoring indicators. The results showed the silicon protein modified by levodopa fixing preferably, having a higher reaction activity and a large detection limit, though the amplitudes were small.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第4期215-220,共6页
Biotechnology Bulletin
基金
天津市自然科学基金重点项目(12JCZDJC22400)
