摘要
目的探讨新型玻璃化冷冻法对人卵巢组织超微结构的保存效果,尤其是对卵巢间质细胞的保存效果。方法收集2007年6月-2009年1月在我院行妇科手术的患者卵巢组织共8例,使用新型玻璃化冷冻法和慢速冷冻法保存,比较两种冷冻方法对于始基卵泡卵母细胞、颗粒细胞、卵泡周围间质细胞超微结构保存效果。结果对于始基卵泡卵母细胞和颗粒细胞,发现两种冷冻方法之间无显著差异(P>0.05);在间质细胞保存中,新型玻璃化冷冻法与慢速冷冻法相比较,正常间质细胞百分率增加(P<0.05)。结论新型玻璃化冷冻法与慢速冷冻法相比能更好地保存卵泡周围间质细胞,在深低温保存人卵巢组织中具有较广阔的应用前景。
Objective To investigate the effect of ultrastructure on human ovarian tissue cryopreservation with a novel needle immersed vitrification （NIV） method. Methods Human ovarian cortical tissues were collected from eight patients who underwent surgery in our hospital between June 2007 and January 2009. The cortical tissues were cryopreserved using vitrification and slow freezing method. The effects were investigated by ultrastructure of oocyte, granulosa cell and stromal cells in primodial follicle using electron microscope. Results No significant difference of normal ultrastructure of oocyte and granulosa cell was observed between NIV and slow freezing group （P 〉 0.05）. The proportion of normal ultrastructure of stromal cells in NIV group was higher than that in slow freezing group （P 〈 0.05）. Conclusions The NIV method can improve the preservation of the ultrastructure of stromal cells in primodial follicle. The NIV method has potential advantages for research and clinical use in human ovarian tissue cryopreservation.
出处
《华西医学》
CAS
2013年第4期567-570,共4页
West China Medical Journal
