摘要
采用聚丙烯酰胺凝胶电泳 ,对商城肥鲵和豫南小鲵 7种组织的酯酶 (EST)、过氧化物酶 (POD)同工酶谱系进行研究。结果表明 :小鲵科动物EST、POD存在较大的组织特异性 ,但没发现性别差异 ;商城肥鲵与豫南小鲵同一组织对应的有些EST、POD酶带的迁移率不同 ,同一组织中有些酶带的表达也不同。因而 ,同工酶的表达与组织功能相一致 。
Comparative study on the isozyme of Esterase and Peroxidase patterns in skin, muscle, spleen, stomach,intestine,liver,lung of Pachyhynobius shangchengensis and Hyno\|bius yunanicus was carried out to probe into their distribution by PAGE. The results showed that their EST and POD were tissue\|specific and no difference in sex. There are differences in some bands and their Rfs of EST and POD in the counterparts tissues between two salamanders. Therefore, the expressions of isozymes was accorded with the functions of tissues, and then specific isozymes can be used as the taxonomic and evolutional basis for animals.
出处
《动物学杂志》
CAS
CSCD
北大核心
2000年第5期19-23,共5页
Chinese Journal of Zoology
基金
河南省自然科学基金!(No .99180 0 14)
河南省动物学重点学科基金
关键词
同工酶
酯酶
过氧化物酶
商城肥鲵
豫南小鲵
Pachyhynobius shangchengensis
Hynobius yunanicus
EST
POD
Isozyme
