摘要
根据GenBank上已登录的拟南芥、苹果杂交种、草莓、桃、沙梨的栽培种美人酥、葡萄、柑橘和玉米的查尔酮异构酶基因蛋白质氨基酸残基序列,应用生物软件对其组成成分、导肽、信号肽、跨膜结构域、疏水性与亲水性、蛋白质二级和三级结构以及功能域进行预测和分析。结果表明:8种植物的CHI不具有导肽,不存在信号肽,不具有跨膜结构,推测CHI可能定位于细胞质基质中发挥功能,多肽链总体表现为亲水性;有α-螺旋、β-转折、无规则卷曲和延伸链4种二级结构,除了无规则卷曲是桃的最主要二级元件外;其余7种植物均是α-螺旋为最主要的结构元件;8种植物可能均有1个查尔酮异构酶活性部位。
The residual amino acid sequences of CHI of Arabidopsis thaliana,Pyrus pyrifolia cultivar Meirensu,Malus hybrid cultivar,Prunus persica,Vitis vinifera,Fragaria×ananassa,Citrus sinensis,Zea mays were adopted from GeneBank.The composition of the residual amino acid sequences,leader peptide,signal peptide,trans-membrane topological structure,hydrophobicity / hydrophilicity,the secondary structure,the tertiary structure and functional domains of the proteins were predicted and analyzed.The results showed that CHI of 8 plant species was a non-transmembrane protein without leader peptide and signal peptide.It was supposed that CHI was positioned to the cell matrix to function directly,the polypeptide chain was generally hydrophobic.There were 4 types of secondary structure including α-helix,β-turn,random coil and extended stand.Except for that the irregular coil was the main secondary motif of Prunus persica,α-helix was the most principal structure motif of the rest 7 plant species.All the eight plant species possessed a chalcone isomerase active site.
出处
《西南林业大学学报（自然科学）》
CAS
2013年第2期88-95,F0003,共9页
Journal of Southwest Forestry University：Natural Sciences
基金
西南林业大学森林培育与经营学科(500961)资助
国家林业局重点实验室开放基金(BC2010F08)资助
西南林业大学科研启动基金项目资助
关键词
查尔酮异构酶
生物信息学分析
蛋白质功能预测
chalcone isomerase
bioinformatics analysis
functional prediction of protein
