摘要
采用生物信息学的方法和工具对已在GenBank上注册的花生、鹰嘴豆、合欢、车前草、沙梨、辣椒等植物的防御素的核酸和氨基酸序列作了分析,并对其组成成分、翻译后修饰、导肽、跨膜结构域、疏水性/亲水性、蛋白质二级结构和功能结构域进行预测和推断的结果表明:此类蛋白可能是一具有信号肽的分泌型蛋白,α-螺旋和不规则卷曲是蛋白质二级结构中量最大的结构元件,β-转角和延伸链散布于整个蛋白质中,包含一个gamma-thionin功能结构域。
By bioinformatic methods and tools, defensin genes as well as the composition of amino acid sequences, post-translational modification, hydrophobicity or hydrophilicity, transmembrane ability, subcellular localization, secondary structure and functional domains of sucrose synthase in Arachis diogoi, Cicer arietinum, Tephrosia platycarpa, Plantago major, Pyrus pyrifolia, and Capsicum annuum were analyzed and predicted. The results demonstrated that defensins is a sort of secretory proteins containing signal peptide. The main motifs of predicted secondary structure of defensin are alpha helix and random coil, beta turn and extended strand are spreaded in the whole secondary structure. Defensins contain a functional domain--gamma-thionin.
出处
《植物生理学通讯》
CSCD
北大核心
2008年第1期25-32,共8页
Plant Physiology Communications
基金
陕西教育学院重点科研项目(06KJ013)。
