摘要
采用生物信息学方法和工具分析19种不同植物中的黄酮醇合成酶基因的核苷酸序列及编码氨基酸序列,对其理化特性、功能结构域、亲/疏水性、二级结构、信号肽、跨膜结构域以及分子系统进化关系等进行预测和推断,并构建分子进化树。结果表明,不同植物FLS基因的开放阅读框全长在1.0 kb左右,编码蛋白含330余个氨基酸,呈酸性,是一个没有任何信号肽、转运肽和跨膜结构域的亲水性蛋白。不同植物FLS氨基酸序列包含有黄酮醇合成酶功能域,而且α-螺旋和不规则盘绕是其蛋白质二级结构的最大量结构元件。19个植物FLS在分子进化树中主要聚成3小支,来源于同科植物的FLS首先聚在一起,再与其他物种FLS聚在一起,进化分析在一定程度上反映了这些物种FLS的进化关系。
In this study, the parameters of flavonol synthase from 19 plants, including physicochemical properties, functional domains, hydrophobicity or hydrophilicity, secondary structure, signal peptide, transmembrane regions, were analyzed and predicted by using bioinformaties methods and software, and the phylogenetie tree of FLS was constructed. The results revealed that the ORF of FLS genes contained about 1 000 nucleic acids and encoded approximate 330 amino acids, and the coding proteins were hydrophilic and acid protein with none signal peptide, transit peptide and trnasmembrane region. A flavonol synthase functional domain was detected in the amino acid sequences, and a-helix and random coil were the dominant motifs in their secondary structure. In the phylogenetic tree, 19 FLSs mainly clustered into three groups, and the ones sourced from the same family were firstly assembled together. The phylogenetie analysis illustrated the evolutionary relationship among the flavonol synthase from 19 plants.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第13期140-143,151,I0001,共6页
Guangdong Agricultural Sciences
基金
国家自然科学基金(21375029)
关键词
黄酮醇合成酶
生物信息学
功能分析
结构预测
flavonol synthase
bioinformatics
functional analysis
structure prediction
