摘要
采用RT-PCR的方法,克隆了野桑蚕Bombyx mandarina Ras1基因,获得了其cDNA序列.该序列长640bp,含有1个579bp的完整开放阅读框,有2个外显子,1个内含子,编码1个由192个氨基酸残基组成的蛋白质,其蛋白质的分子量和等电点分别为21 800和6.24.推导的氨基酸序列与其它物种Ras1基因相应氨基酸序列有较高的同源性,该序列具有它们的Ras1基因所共有的典型特征.组织特异性表达分析表明该基因在野桑蚕的丝腺、脂肪体、表皮、中肠和马氏管中均有表达.这些结果为进一步研究野桑蚕Ras1基因的功能奠定了分子基础.
The complemental deoxyribonucleic acid （cDNA） of Bornbyx mandarina Ras 1 gene was cloned by reverse transcription-polymerase chain reaction（RT-PCR）. The results showed that the cDNA with 640 bp in length contained an open reading frame （ORF） of 579 bp which encoded 192 amino acid residues, contains 2 exons and 1 intron, and a predicted molecular weight of 21 800 and isoelectric point of 6.24. The deduced amino acid sequence had a high identity to the reported sequence of Rasl from other species and shared the typical structural features of Ras1 from other insects. The Rasl was expressed in malpighian tubule, fat body, silk gland, epidermis, and midgut of B. mandarina by RT-PCR analysis. These results provide molecular basis for further studying the function of Rasl gene in B. mandarina.
出处
《华中师范大学学报（自然科学版）》
CAS
北大核心
2013年第2期250-253,共4页
Journal of Central China Normal University：Natural Sciences
基金
河南省教育厅自然科学基金项目(2010A230016)
周口师范学院博士科研启动基金资助项目
周口师范学院校级重点学科建设资助项目
关键词
野桑蚕
Ras1基因克隆
序列分析
转录表达
Bombyx mandarina
Ras1 gene cloning
sequence analysis
transcriptional ex- pression
