摘要
Ras蛋白是一个分子质量为21kD左右的单体GTP酶,具有两种构象:GTP结合构象(Ras.GTP)及GDP结合构象(Ras.GDP),这两种构象在一定条件下可发生互变。由生长因子介导的Ras信号传导途径是诸多信号途径中与细胞增殖、分化密切相关的重要信号途径。受体型TPK/Ras/MAPK信号转导途径是是目前研究的最为清楚的受Ras蛋白调节的信号传导途径,该途径包括受体型酪氨酸蛋白激酶(RTK)、接头蛋白、鸟苷酸释放因子(GNEF)、Ras蛋白以及MAPK级联反应体系。目前,TPK/Ras/MAPK信号转导途径在秀丽杆线虫(Caenorhabolitis elegans中研究的最为清楚:Ras信号途径对于许多发育进程是必需的,包括阴门、子宫、交合刺、P12以及排泄管细胞的诱导分化;控制着性肌原细胞迁移、轴突导向;对细胞减数分裂粗线期具有促进作用。对C.elegans的研究加深了对TPK/Ras/MAPK信号途径结构、突变体表型以及与其他信号途径的互作的了解,将会促进Ras信号途径对植物寄生线虫调控作用的研究。
Ras protein is a single GTPase that cycles between an inactive GDP-bound state,and an active GTP-bound state, and its molecule weight is about 21 kD. TPK/Ras/MAPK signal transduction pathway regulated by Ras protein has been well studied,including receptor tyrosine kinase（RTK） ,adaptor protein, guanine nucleotide exchange factors（ GNEF）, Ras protein and mitogen activated protein kinase（MAPK） cascade reaction system. In Caenorhabditis elegans, it is clear that TPK/Ras/MAPK pathway is required for multiple developmental events, including induction of vulval, uterine, spicule, P12 and excretory duct cell fates, control of sex myo- blast migration, axon guidance, and promotion of germline meiosis. Studies of C. elegans have provided insight into the basic framework of this RTK/Ras/MAPK signaling pathway, the phenotype of its mutants, and how interacts with other signaling pathways,all of which would promote the researches on plant parasite nematodes.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第8期32-37,共6页
Biotechnology Bulletin
基金
国家自然科学基金(30571261)
"十一五"国家高技术研究发展计划课题(2006AA10Z1119)
