摘要
应用免疫组织化学和生物统计分析相结合的方法,对花胫绿纹蝗Aiolopus tamulus（Fabricius）和疣蝗Trilophidia annulata（Thunberg）配子发生过程中原癌基因c-kit特异表达进行了比较研究。结果表明：（1）精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c-kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;（2）卵子发生过程中,第1-6阶段卵母细胞中有不同程度的c-kit蛋白特异性表达,但随着卵黄发生的开始逐渐消失;（3）2种蝗虫配子发生过程中c-kit表达存在种间差异。
The special expression of c-kit protein in gametogenesis of Aiolopus tamulus （Fabricius） and Trilophidia annulata （Thunberg） collected from Siping, Jilin, Northeast China was studied by using immunohistochemistry and biostatistics. The results showed that： （1） In spermatogenesis, there were different expression levels of c-kit protein in spermatogonia, primary spermatocytes, second spermatocytes and mature sperms, and the biggest positive granules were found at the end of spermary; （2） In oogenesis, there were different expression levels of c-kit protein from the 1st to 6th stage of oocytes, and the positive granules gradually disappeared in vitellogenesis; （3） The expression of c-kit protein in gametogenesis was significantly different between the two species.
出处
《昆虫学报》
CAS
CSCD
北大核心
2007年第5期441-447,共7页
Acta Entomologica Sinica
基金
陕西省自然科学基金项目(2004C107)
关键词
花胫绿纹蝗
疣蝗
配子发生
C-KIT蛋白
特异性表达
Aiolopus tamulus
Trilophidia annulata
gametogenesis
c-kit protein
special expression
