摘要
目的:探讨阿尔茨海默病模型大鼠海马PKA的变化以及电针对其的影响。方法:将40只SD大鼠按随机数字表分为4组,即正常组、假手术组、模型组、电针组。采用双侧海马一次性注射聚集态Aβ25-35制备AD大鼠模型,电针组选取双侧"肾俞"穴、双侧"内关"穴和"大椎"穴,接G6805-Ⅱ型电针治疗仪,选用2 Hz连续波,强度为1 mA,通电20 min,每天1次,每周6次,共治疗2周。治疗结束后,采用免疫组化法检测各组大鼠海马PKA表达的变化,并进一步分析电针对其的影响。结果:经图像分析,模型组大鼠海马CA1、CA3区PKA的表达明显低于假手术组,差异有显著性(P<0.01);与模型组相比,电针组大鼠海马CA1、CA3区PKA表达明显增多(P<0.05)。结论:电针可能通过增强海马PKA的表达,改善LTP,提高AD大鼠的学习记忆能力。
Objective ：To explore the changes of protein kinase A in the hippocampus of rats with Alzheimer＇s disease and observe the effect of electro- acupuncture therapy on the hippocampus. Methods ：40 Male SD rats were randomly di- vided into 4 groups in average：the normal group, the sham group, the model group and the EA group. By utilizing con- densed Aβ25 -32 in one- time injection of bilateral hippocampus to induce AD model rats ,the research selected bilat- er- alm Shenshu （ BL2,3 ）, bilat - eralm Neiguan （PC6） and Dazhui （ GV14 ） acupoints in EA group. G6805 - Ⅱ type of electroacupuncture treatment device was connected to needdles,and then selected the frequency of 2Hz continus wave（ CW）, the intensity of 1 mA. The therapy lasted 20min, once a day,6 times a week, a total of 2 weeks treatment. The expression of PKA protein in hippocampus was assayed by immunohistochemistry. Results ： Compared with the sham group, the ex- pression of PKA in hippocampus of the model group was lower, and the difference was significant （P 〈 0.01 ）;compared with the model group, the expression of PKA in hippocampus of the EA group was significantly increased （ P 〈 0. 05 ）. Conclusion：Its mechanism may be achieved through increasing the expression of PKA in the hippocamPus and enhancing LTP in order to improve the learning - memory capacity of rats with AD.
出处
《中华中医药学刊》
CAS
2013年第4期731-733,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81273864)
湖北省教育厅科研资助项目(Q20121607)
关键词
电针
AD
海马
PKA
electro - acupuncture
Alzheimer
disease
hippocampus
protein kinase A
