摘要
目的:探讨中药寒热药性与所含多糖成分的相关性。方法:取寒性、热性植物药各10味,提取并精制所含多糖,用三氟乙酸彻底水解成单糖,加入1-苯基-3-甲基-5-吡唑啉酮进行衍生化反应,测定其单糖HPLC图谱,并利用主成分-线性判别分析研究结果。结果:主成分-线性判别分析完全实现了对20味植物药寒热药性准确判别。结论:中药寒热药性与所含多糖成分存在明显相关性,多糖是中药药性的物质基础之一;主成分-线性判别可对中药药性进行判别,且判别准确率高。
Objective：To discuss the correlation between cold-heat potencies and polysaccharides. Methods：Extracting polysacch- arides from 10 kinds of cold botanical Chinese medicinal and 10 hot botanical Chinese medicinal. The polysaccharides were hydrolyzed into monosaccharides with trifluoroacetic acid. The monosaccharide derivatives obtained with 1-phenyl-3-methyl-5-pyr- azolone were separated by reverse-phase high performance liquid chromatography（RP-HPLC） using a developed gradient elution process. The results were analyzed by principal components-linear discriminant analysis（PC-LDA）. Resuhs：PC-LDA could accu- rately distinguish cold or heat potencies of 20 kinds of Chinese medicinal. Conclusion：There is a significant correlation between cold-heat potencies and polysaccharides. The polysaccharides is one of the material basis potencies of Chinese medicinal. The PC-LDA analysis can distinguish cold or heat potencies.
出处
《山东中医药大学学报》
2013年第2期156-159,共4页
Journal of Shandong University of Traditional Chinese Medicine
基金
国家重点基础研究发展计划("973"计划)项目(编号:2007CB512601)
关键词
多糖
HPLC
寒热药性
主成分-线性判别分析
polysaccharides
HPLC
cold-heat potencies
principal components-linear discriminant analysis
