摘要
用复方６温热药（热Ⅰ：附子、干姜，热Ⅱ：党参、黄芪）治疗寒证大鼠。取大鼠垂体，用ＡＢＣ免疫组化方法，观察促黄体激素、促甲状腺激素和促肾上腺皮质激素细胞的变化。结果表明：寒证时，３种垂体激素均有明显增高，给予热药治疗后各垂体激素大量释放。其中以ＡＣＴＨ释放尤为显著。两种复方温热药作用相同。通过对３种激素的靶器官（睾丸、甲状腺、肾上腺）的形态学观察，均未发现有明显改变，提示寒凉及温热药的剂量及给药时间尚未引起其靶器官的器质性改变。与对照组比较Ｐ＜０．００５．＊与对照组和寒证组比较Ｐ＜０．００５△与热Ⅰ方组比较Ｐ＜０．９０１ＴＳＨ免疫反应强阳性细胞明显的增多（图２）。然而在热药治疗以后，成强阳性反应细胞的数目减少。但其减少的程度没有ＡＣＴＨ细胞的变化那么明显。计数对照组和实验组不同免疫反应强度的ＴＳＨ细胞数的结果见表２。２．３ＬＨ细胞的免疫组织化学变化用兔抗羊ＬＨ血清所显示的促黄体激素细胞在数量、形态及分布上均与ＴＳＨ细胞相一致；给与寒性药及热性药治疗后细胞免疫组织化学的改变亦与ＴＳＨ细胞变化相同，故未加以计数。２．４各靶器官ＨＥ染色变化ＡＣＴＨ、ＬＨ、ＴＳＨ作用的靶器官分别为肾上腺束状带、睾丸的间质细胞及甲状腺?
The experimental rats were fed with cold-natured medicines (Zhimu, Shigao) for three weeks to made a 'cold ' syndrome model. After that, they were treated with two kinds of heat-natured medicines (I-Fuzi, Ganjiang: Ⅱ-Dangshen, Huangqi) respectively. After 5 and 7 days , the pituitary, thyroid, adrenal, and testis were taken.The specific anti-LH, antiTSH adn anti-ACTH sera were used in the avidin-biotin-complex (ABC) immunohistochemical method to observe the changes of ACTH, LH and TSH cells. It was observed that the immunohistochemical reaction of the three kinds of pituitary hormone-secreting cells increased obviously in rats with 'cold' syndrome and weakened after treated with heat-natured medicines. Among the three kinds of cells the change of ACTH cell was most remarkable.The changes of pituitary hormone-secreting cells treated with heat-natured medicines I were similar to those of heat-natured medicines Ⅱ. We also observed the morphologic changes of the three target organs. It was found that there was no obvious change in them and it might indicate that the amount of hormone was not enough to cause morphologic changes but only the functional ones.
