摘要
目的 :观察维拉帕米 (verapamil,VP)对三磷酸肌醇 (IP3)引起的大鼠肝细胞钙释放激活的钙电流 (ICRAC)的影响。方法 :采用标准全细胞膜片钳技术。结果 :IP3 激活的ICRAC的峰电流为 (- 0 .2 6± 0 .0 8)nA(n =7) ;5 μmol/L维拉帕米对ICRAC的抑制率为 30 .9%± 7.8% (从 - 0 .2 5± 0 .10nA到 - 0 .17± 0 .0 5nA ,n =5 ,P <0 .0 1)。结果 :维拉帕米能有效抑制IP3 引起的ICRAC。
Aim and Methods:Whole-cell patch clamp techniue was employed to investigate the influence of verapamil on calcium release-activated calcium currents(I CRAC ) caused by 1,4,5-triphosphate (IP 3) in rat hepatocytes. Results: The peak amplitude of IP 3-activated I CRAC was -0.26±0.08 nA( n =7); The inhibitory rate of 5 μmol/L verapamil on I CRAC was 30.9±7.8%(from -0.25±0.10 nA to -0. 17±0.05 nA, n =5, P <0.01). Conclusion: The results showed that I CRAC was a calcium current activated by depletion of intracellular calcium stores caused by IP 3. Verapamil could protect hepatocytes from calcium overload via the inhibition of I CRAC .
出处
《中国应用生理学杂志》
CSCD
2000年第3期265-267,共3页
Chinese Journal of Applied Physiology
基金
国家自然科学基金!资助课题 (3940 0 138)
