摘要
采用不同的转化液制备大肠杆菌(Escherichia coli)JM109感受态细胞,并进行pUC18质粒的转化,考察细菌生长状态、转化液、转化的热激时间和转化后所用培养基对转化率的影响。结果表明,菌液A600 nm为0.705时采用TB转化液制备感受态细胞,在42℃热激45 s,转化后采用SOC培养基振荡培养,转化效率最高,可达8.59×108CFU/μg质粒。
Competent cells of Escherichia coli JM109 were prepared by different transformation liquid,and pUC18 was transformed into the competent cells.The effects of different growth state of bacteria,transformation liquid,heat shock time and culture medium after heat shock on transformation efficiency were studied.The results showed that the highest transformation rate could be obtained by preparing the competent cells by TB transformation liquid when bacteria A600 nm was 0.705,heat shocked at 42 ℃ for 45 s and then culturing in SOC medium.The transformation efficiency could reach to 8.59×108 CFU/μg plasmid.
出处
《湖北农业科学》
北大核心
2012年第21期4902-4904,共3页
Hubei Agricultural Sciences
