摘要
目的观察麻醉复苏过程中大鼠皮质脊髓束（CST）神经电信号放电频率、波幅、峰间期（isi）等的变化，描述并分析麻醉复苏期大鼠CST神经电信号的特征差异。方法在复合麻醉剂Chlorpent麻醉后SD大鼠的脊髓L节段处的CST内插入微电极，分别在麻醉后1h、2h、3h应用神经信号采集处理系统Cerebus记录大鼠CST神经电信号，利用神经信号分析软件NeuroExplorer及Off-lineSorter分析并比较CST神经电信号放电频率、波幅、ISI的变化。结果与麻醉后1hf麻醉状态）比较，麻醉后2h、3h（复苏状态）大鼠的CST自发放电频率显著增加，波幅明显减小[（640．13±126．01）UVus（200．44＋19．53）uⅣ、（144．49±22．52）uV]，差异有统计学意义护〈0．05）。ISI值从较为集中变化为较为分散。结论麻醉复苏过程中大鼠的CST自发放电在放电频率、波幅、ISI等神经电信号的特征方面存在明显差异。
Objective To observe the amplitude, discharge frequency and interspike interval （ISI） changes of the electrophysiological signals from corticospinal tract （CST） of adult rats at palinesthesia stage after anesthesia to study their characteristic differences. Methods The microelectrodes were plugged into CST at T8 spinal section after the Sprague-Dawley rats were anesthetized with Chlorpent. The neuro-electrical signals were identified and recorded from CST by means of Cerehus system at 1, 2 and 3 h after anesthesia, respectively. The characteristics of the recorded signals, including the amplitude, discharge frequency and ISI, were described with the help of Off-line sorter and Neuroexplorer softwares. Results The discharge frequency of the neuro-electrical signals in CST at palinesthesia stage （2 and 3 h after anesthesia） obviously increased as compared with that at narcotism （1 h after anesthesia）. The wave amplitude decreased gradually following the times [（640.13±126.01） uV vs （200.44±19.53） uV and （144.49±22.52） txV]. The ISI changed from localization to dispersion. Conclusion Conspicuous characteristic differences of electrophysiological signals from CST of rats in palinesthesia stage after anesthesia, including the wave amplitude, discharging frequency and ISI, are noted.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2013年第4期376-380,共5页
Chinese Journal of Neuromedicine
基金
国家自然科学基金（30707005）
国家自然科学基金（30970996）
关键词
皮质脊髓束
神经电信号
麻醉复苏
特征差异
Corticospinal tract
Electrophysiological signal
Palinesthesia stage
Characteristic difference
