摘要
1目的 探讨降钙素 (CT)基因高度甲基化与恶性血液病预后的关系。 2方法 采用多聚酶链反应(PCR)技术 ,观察了 46例 CT基因高度甲基化阳性的恶性血液病病人完全缓解 (CR)后 CT基因的动态变化。 3结果 CT基因高度甲基化为阳性的 46例恶性血液病病人 ,CR后 30 d,有 32例病人仍为阳性 ,其余 14例转阴 ;CR后6 0 d,32例阳性病人中又有 5例转为阴性 ,而 14例阴性病人有 1例又转为阳性。随访发现 2 8例 CT基因高度甲基化阳性的病人有 19例在 CR后 6 0 d～ 1年内复发 ,复发率为 6 7.86 % ;18例阴性病人仅有 6例在同期内复发 ,复发率为 33.33% ,两组比较差异有极显著性 (χ2 =15 .84,P<0 .0 0 1)。 4结论 CT基因高度甲基化与恶性血液病病人的预后密切相关 ,且可作为预测疾病复发、指导临床治疗的可靠指标之一。
Objective To study the relationship between calcitonin (CT) gene hypermethylation and the prognosis of malignant hematological disorders.\ Methods\ Hypermethylations of CT gene in 46 malignant hematological disorders were observed after complete remission (CR) by the technique of polymerase chain reaction (PCR).\ Results\ Of the 46 patients with CT gene hypermethylation (positive), 32 remained positive 30 days after CR, 14 reverted to negative 60 days after CR, another 5 patients of the 32 positive patients reverted to negative, while 1 of the 14 negative patients became positive. In the follow up study of 60 days-1 year after CR, 19 of the 28 positive patients relapsed, the relapse rate was 67.86%, 6 of the 18 negative patients relapsed, the relapse rate was 33.33%. There was significant difference in relapse rate between the two groups ( χ 2=15.84, P < 0.001 ).\ Conclusion\ CT gene hypermethylation correlates with the prognosis of malignant hematological disorders, it may be an useful target for predicting relapse and directing the treatment. [
出处
《青岛大学医学院学报》
CAS
2000年第4期243-244,共2页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省教委科研基金!资助课题 ( J90 K0 5 )
关键词
降钙素基因
甲基化
预后
恶性血液病
calcitonin gene
methylation
malignant hematological disorders
prognosis
relapse
