摘要
目的 :为了证实 TNF- α对局部脑缺血 /再灌流区 ICAM- 1m RNA表达的影响 ;方法 :运用原位杂交技术对 6 0只雄性 SD大鼠进行了研究。结果 :局部脑缺血 /再灌流诱导脑微血管和毛细血管以及局部炎症细胞 ICAM- 1m RNA的表达 ,其表达发生于脑缺血 1h/再灌流 2 h,再灌流 8h达到高峰。TNF- α明显的加强脑缺血区 ICAM- 1m RNA的表达 ,其作用在脑缺血 1h/再灌流 2 h至 8h均较明显。再灌流 4h,TNF- α的诱导作用最显著。结论 :局部脑缺血 /再灌流诱导 ICAM- 1m RNA 表达 ,TNF- α对其表达具有明显的促进作用。提示TNF- α通过调节 ICAM- 1m RNA的表达参与脑缺血
Objective:To test the effect of TNF α on ICAM-1 mRNA expression in ischemic at rat. Method: In situ hybridization histochemistry was used in the study. Results: ① Focal ischemia/reperfusion-induced ICAM-1 mRNA expression occurred on the microvascular and capillary endothelial cells, and focal inflammatory cells at 1h ischemia/2h reperfusion, peaked at 8h reperfusion. ② TNF α induced strongly ICAM-1 mRNA expression in the ischemic cerebral area during 2h-8h reperfusion, and the strongest induce-effect was at the time point of 4h reperfusion. Conclusion: Focal cerebral ischemia/reperfusion induced ICAM-1 mRNA expression in the ischemic brain, and TNF α accelerated ICAM-1 mRNA expression. This result indicated that TNF α participated in the pathogenesis of ischemic cerebral injury.
出处
《解剖科学进展》
CAS
2000年第3期240-243,共4页
Progress of Anatomical Sciences
