摘要
通过有机溶剂提取、柱色谱分离和花色素反应鉴定得到棉花缩合单宁。将不同浓度的棉花缩合单宁和 Bt毒蛋白拌入人工饲料对棉铃虫幼虫进行剂量反应试验 ,发现棉花缩合单宁和 Bt毒蛋白之间有一定的拮抗作用。采用高效液相色谱法测定转 Bt基因棉花缩合单宁的的变化 ,结果表明3叶期到花铃期转 Bt基因棉花的缩合单宁含量显著低于受体品种 ,在一些组织器官中缩合单宁含量减少达 30 % ,说明 Bt杀虫蛋白表达会影响到棉花中缩合单宁的合成。
Different dosage combination of condensed tannin were extracted and purified roughly from cotton plants with organic reagents,and column chromatograph and HCL/n butanal hydrolysis reaction methods, and Bt crystal protein of CryⅠA C were mixed into artificial diets of cotton bollworm Helicoverpa armigera (Hübner)for determining the toxicity interactions of the two materials on the larvae.It was found that there was nearly significant antagonism between condensed tannin and Bt crystal protein,causing the reduce of larval mortalities. In association with the results of series HPLC analyses that the contents of condensed tannin in Bt transgenic cotton from three leaf stage to flowering bolling stage were significantly lower, even 30% lower in some organs than those in non Bt transgenic cotton (of the same variety). It was indicated that owing to the antagonism between condensed tannin and Bt crystal protein, the transference of Bt gene into cotton plant has reduced its production of condensed tannin.
出处
《棉花学报》
CSCD
北大核心
2000年第6期294-297,共4页
Cotton Science
基金
国家自然科学基金资助项目! ( 39770 4 98)
