摘要
为明确苜蓿中被蓟马为害后诱导的次生代谢物质含量的变化,以抗蓟马苜蓿R-1和感蓟马苜蓿I-1为材料,以每枝条0头为对照,研究牛角花齿蓟马不同虫口密度(每枝条1、3、5、7头)下苜蓿叶片中酚类物质和木质素含量的变化.结果表明:蓟马为害7 d时,R-1和I-1叶中总酚、单宁和缩合单宁含量随虫口密度增大均升高,简单酚含量无显著差异;木质素含量显著高于对照.为害14 d时,R-1和I-1叶中总酚、单宁和缩合单宁含量随虫口密度增大均升高,简单酚含量无显著差异;木质素含量升高,且每枝条7头密度下显著高于对照.为害21 d时,R-1和I-1叶中总酚、单宁和木质素含量随虫口密度增大均升高,在每枝条7头密度下达到最高;R-1简单酚含量变化不显著,I-1简单酚含量显著升高.R-1和I-1叶中缩合单宁含量与对照无显著差异.抗感苜蓿中酚类物质与木质素含量在蓟马为害后均升高,R-1总酚、单宁和木质素含量的增加率均高于I-1.表明蓟马为害对苜蓿植株的总酚、单宁及木质素含量具有诱导效应,可以作为苜蓿抗蓟马的评价指标.
In order to investigate the changes of secondary metabolites content of alfalfa induced by thrips Odontothrips loti damaging, two alfalfa strains, one resistant to thrips （R-1） and the other susceptible to thrips （ I-1 ） , were chosen to measure the phenols and lignin contents of alfalfa leaves under infestation with thrips at different densities （0, 1, 3, 5, 7 thrips · branch-1 , and 0 thrip · branch-1 as control）. After infestation 7 days, the polyphenols, tannin and condensed tannin con- tents increased in both leaves of R-1 and I-1 with the increasing thrips density, the simple phenols content had no significant difference, while the lignin content increased significantly compared with the control. After infestation 14 days, the polyphenols, tannin, condensed tannin and lignin con- tents in both leaves of R-1 and I-1 increased obviously with the increasing thrips density, while the simple phenols content had no significant difference. The lignin content increased significantly, and was significantly higher under 7 thrips · branch-1 than under the control. After infestation 21 days, the polyphenols, tannin, and lignin contents in both leaves of R-1 and I-1 increased obviously with the increasing thrips density, and were the highest under 7 thrips · branch-1. Simple phenols con- tent of I-1 strain was increased significantly, but that of R-1 strain had no significant change. The condensed tannin content in both leaves of R-1 and I-1 was not obvious compared with the control. Phenols and lignin contents in R-1 and I-1 leaves increased obviously after thrips infestation, and the polyphenols, tannin and lignin infestation had inductive effects on evaluate the resistance of alfalfa. contents increased faster in R-1 strain than in I-1 strain. Thrips phenols and lignin contents of alfalfa, which could be used to
出处
《应用生态学报》
CAS
CSCD
北大核心
2014年第6期1688-1692,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(31260579)
甘肃农业大学草业生态系统教育部重点实验室开放课题(CYZS-20110009)
教育部高校博士学科点基金项目(20106202110003)
国家现代农业产业技术体系建设专项(CARS-35)资助
