摘要
从广东某硫铁矿山酸性废水中分离到一株嗜酸性细菌,经形态观察、生理学和近全长16S rRNA基因分析鉴定为氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans,简称A.ferrooxidans),命名为Z1。该菌与GenBank中菌株A.ferrooxidansCMS等处在系统发育树的同一分支,16S rRNA基因序列相似度均为99%。菌株Z1生长的最适pH值和温度分别为2.25和30℃,对数生长期处于第18～30小时,Fe2+平均氧化速率达到0.2307 g/(L.h),经驯化后能耐受15 g/L的废旧线路板金属富集体。浸出实验结果表明,在起始pH值为2.25、起始Fe2+浓度为9 g/L、接种量为10%、金属富集体投加量为15 g/L的条件下,菌株Z1能在62 h内浸出废旧线路板中99.3%的铜。与生物浸出效果类似,过滤除菌的滤菌液处理能在86 h内浸出96.0%的铜。而不接种上述细菌的9K培养基无菌浸出对照134 h铜的浸出率仅为61.3%。因此,菌株Z1可作为浸出废旧线路板中有价金属的潜在有效菌株。
An acidophilic bacterium named as Z1 was isolated from the acid mine drainage of a pyrite mine in Guangdong. It was identified as AcidithiobaciUus ferrooxidans （A. ferrooxidans ） genus by morphology, physiology and near full-length 16S rRNA gene analysis. The strain Z1 and the strains such as A. ferrooxidans strain CMS in GenBank were in the same branch of the phylogenetic tree. The 16S rRNA gene sequence of the strain Z1 had 99% sequence simi- larity to A. ferrooxidans strain CMS. The optimum growth pH value and temperature, and the logarithmic phase of strain ZI were 2.25 and 30℃, and from 18 h to 30 h, respectively. The average ferrous oxidation rate of the Z1 reached 0.2307 g/（L · h）. The adapted strain Z1 could tolerate 15 g/L metal concentrates of waste printed circuit boards. The results of bioleaching experiments showed that 99.3% copper could be mobilized from metal concentrates in 62 h under the condi- tions of initial pH of 2.25, 9 g/L of initial Fez＋ , 10% of inoculation quantity, 15 g/L of metal concentrates. Similarly, the filter-sterilized solution could leach 96.0% copper from metal concentrates in 86 h. However, the copper mobilization efficiency of abiotic leaching using only 9K liquid medium without inoculating strain Z1 was just 61.3% at 134 h. Therefore, the isolated strain Z1 can be used as a potential bacterium to bioleach metals from waste printed circuit boards.
出处
《环境工程学报》
CAS
CSCD
北大核心
2013年第4期1482-1488,共7页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金资助项目(51178191)
教育部新世纪优秀人才支持计划(NCET-11-0166)
广东省科技计划项目(2008B030302004)
关键词
氧化亚铁硫杆菌废旧线路板金属富集体生物浸出
铜
Acidithiobacillus ferrooxidans
waste printed circuit boards
metal concentrates
bioleaching
copper
