摘要
背景:葛根素能抑制椎间盘纤维环细胞凋亡,在一定程度上延缓椎间盘退变。目的:观察最佳浓度葛根素对大鼠颈椎间盘纤维环细胞的保护作用。方法:用不同质量浓度Fas配体(5,20,50μg/L)诱导大鼠颈椎间盘纤维环细胞凋亡,用流式细胞仪Annexin V–FITC-PI双染法检测纤维环细胞凋亡情况,通过MTT法和流式细胞仪观察不同质量浓度葛根素(0.01,0.1,1g/L)对纤维环细胞的保护作用。结果与结论:5μg/LFas配体诱导的纤维环细胞存活率及早期凋亡率与胎牛血清对照组比较差异无显著性意义(P>0.05),20μg/L及50μg/LFas配体组纤维环细胞存活率及早期凋亡率高于胎牛血清对照组(P<0.01),且随着Fas配体质量浓度的增加,大鼠成熟纤维环细胞活性比例逐渐降低,而早期凋亡率逐渐增加。3种浓度的葛根素作用20μg/LFas配体诱导的纤维环细胞,随着葛根素质量浓度的增加,纤维环细胞存活比例逐渐增加,而早期凋亡率逐渐减少(P<0.01)。结果提示:①Fas配体能够诱导大鼠颈椎间盘纤维环细胞凋亡,增加大鼠成熟颈椎间盘纤维环细胞的凋亡率。②3种质量浓度葛根素均有抑制椎间盘纤维环细胞死亡作用,且呈剂量依赖型。③葛根素对大鼠颈椎间盘纤维环细胞有明显保护作用。
BACKGROUND： Puerarin restrains the apoptosis of disc annulus fibrosus cells and delays the degenerationof the intervertebral disc. OBJECTIVE： To explore the protective role of puerarin in rat cervical intervertebral disc annulus fibrosuscells METHODS： Cervical intervertebral disc annulus fibrosus cells were induced by different concentrations of Fas ligand （5, 20, 50 pg/L）. The appropriate concentration of puerarin was detected using Annexin V-FITC-PI double staining to establish an in vitro apoptotic model of annulus fibrosus cells. Changes of annulus fibrosus cells in response to different concentrations of puerarin （0.01, 0.1, 1 g/L） were observed by flow cytometry and MTT method. RESULTS AND CONCLUSION： 5 pg/L Fas ligand had no significant influence on the survival rate and early-apoptotic rate of annulus fibrosus cells （P 〉 0.05）. 20 and 50 IJgtL Fas ligand significantly promoted annulus fibrosus cells apoptosis （P 〈 0.01） in a dose-dependent manner. The 0.01, 0.1 and 1 g/L puerarin could improve the survival rate of Fas ligand-induced annulus fibrosus cells in a dose-dependent manner （P 〈 0.01）. These findings indicate that （1） Fas ligand can induce the disc annulus fibrosus to be in apoptosis status. （2） Puerarin （0.01, 0.1, 1 g/L） can promote the proliferation of disc annulus fibrosus cells in a dose-dependent manner. （3） The apoptosis rate of disc annulus fibrosus cells induced by Fas ligand in vitro can be restrained by puerarin interference.
出处
《中国组织工程研究》
CAS
CSCD
2013年第7期1156-1161,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(30973764)
福建省卫生教育联合攻关计划项目(WKJ2008-2-48)
福建省科技厅国际合作项目(2009I0010)~~
关键词
组织构建
软骨组织构建
颈椎病
葛根素
中药
最佳浓度
剂量依赖
FAS
FASL
细胞凋亡
纤维环细胞
大鼠
国家自然科学基金
组织构建图片文章
tissue construction
cartilage tissue construction
cervical spondylosis
puerarin
Chinese herbs
optimal concentration
dose-dependent
Fas/Fas ligand
cell necrosis
annulus fibrosus cells
rats
the NationalNatural Science Foundation of China
tissue construction photographs-containing paper
