摘要
目的 :建立复方苦参注射液的质量控制标准。方法 :用薄层色谱法对该制剂中当归、苦参进行定性鉴别 ;用 HPL C法测定该制剂中阿魏酸的含量 ,并用 TLC法测定该制剂中苦参碱的含量。结果 :薄层色谱中斑点清晰 ,易于识别 ;阿魏酸在 1.95～ 9.76μg·mg-1范围内呈线性关系 ,r=0 .9980 ,平均回收率为 96.8 ,RSD=0 .3。苦参碱斑点在 2 .16～ 7.5 6μg之间与其吸收度积分值呈线性关系 ,r=0 .9992 ,平均回收率为99.0 ,RSD=0 .61。结论
OBJECTIVE:To establish a quality control standard of compound Radix sopharae flavescentis injecton.METHODS:The Radix angelicae sinensis and Radix sophorae flavescentis in compound Radix sophrae flvescentis injection were identified by TLC.The content of ferulic acid in the injection was determined by HPLC.Also the content of matrine in the injection was determined by TLC-scanning.RESULTS:The spots in the TLC were clear and distinguishable.The linear ranges of ferulic acid was 1.95-9.76 μg.ml [-1] ,r=0.9980.The average recovery was 96.8%,RSD=0.3%.The linear ranges of matrine was 2.16-7.56 μg,r=0.9992(n=6).The average recovery was 99.0%,RSD=0.61%.CONCLUSIONS:These methods can be used for quality control of compound Radix sophrae flavescentis injection.
出处
《基层中药杂志》
2000年第4期12-14,共3页
Primary Journal of Chinese Materia Medica
关键词
复方苦参注射液
阿魏酸
苦参碱
中药
质量标准
Compound Radix sophorae flavescentis injection
ferulic acid
matrine
TLC
HPLC
TLC-scanning
