摘要
建立了测定苦参碱和氧化苦参碱含量的高效毛细管电泳法。 ２０ ｍｍｏＬ／Ｌ的磷酸盐缓冲液（ｐＨ＝８．０）为电泳电解质，电压为３０ｋＶ（运行电流为１００～１１０ｕＡ），检测波长为２１４ｎｍ，内标氨茶碱。苦参碱 ０．１２６～１．２６ ｍｇ／ｍＬ、氧化苦参碱 ０．１０～１．０ ｍｇ／ｍＬ其浓度与峰面积之比呈良好的线性关系，ｒ均为０．９９９８，日内、日间变异系数均小于５．７％，常用２２种药物除地塞米松外其余的对其无干扰。用本法测定了２批苦参提取物，其中苦参碱为（４５．１９±０．０５８）％，（６２．８１±０．１２３）％；氧化苦参碱为（８．７１±０．０３）％，（６．８１±０．０８６）％。结果表明，该方法简单、灵敏，特异性和重现性良好，具有较强的实用价值。
A method has been developed for the determination of matrine and oxymatrine by high performance capillary electrophoresis (HPCE). Aminophylline was used as internal standard. Complete separation was achieved with 20 mmol/L running phosphate buffer (pH=8.0) at a constant voltage of 30 kV (current of 100~ 110 PA) and detected at a wavelength of 214 nm. Good linearity was shown between the concentration and peak area in the concentration range of 0.126-1. 26 mg/mL for matrine and 0.10~ 1.0 mg/mL for oxymatrine, r=0.9998. The relative standard deviation (RSD) for within-day and between-day was less than 5. 7 %. 22 different drugs in common use except dexamethasone showed no interference. Two samples of extract of Sophora flavescens Ait. gave results of (45.19±0.058) % and (62.81±0.123) % for matrine and (8.71±0. 03) % and (6. 81± 0. 086) % for oxymatrine on a trial determination. It seemed that the HPCE method is faster, more convenient and economical as compared with HPLC.
出处
《中草药》
CAS
CSCD
北大核心
1999年第4期261-263,共3页
Chinese Traditional and Herbal Drugs
关键词
高效毛细管电泳
苦参碱
氧化苦参碱
含量检测
high performance capillary electrophoresis (HPCE)
matrine oxymatrine
