摘要
【目的】研究甘精胰岛素(insulinglargine)对游离脂肪酸诱导的β细胞凋亡是否具有保护作用。【方法】不同浓度的甘精胰岛素、普通人胰岛素以及IGF-1与0.3mmol/L棕榈酸分别加入RIN-m细胞培养液中,24h后,采用Hoechst33342染色、流式细胞仪以及ELISA方法检测RIN-m细胞的凋亡。【结果】0.3mmol/L棕榈酸诱导RIN-m细胞凋亡率达42.10%±4.24%,同时加入甘精胰岛素或者普通胰岛素(均为100nmol/L)的凋亡率分别为32.00%±3.08%和35.97%±3.14%,甘精胰岛素对凋亡的抑制作用明显强于普通胰岛素,并呈剂量依赖性。【结论】甘精胰岛素对游离脂肪酸诱导的β细胞凋亡具有呈剂量依赖性的保护作用。
[Objective]To investigate the effects of insulin glargine on apoptosis induced by free fatty acid in pancreatic βcells. [Methods] Apoptosis was determined by Hoechst staining, flow cytometry, and ELISA kit in RIN-m cells incubated by palmitic acid (0.3mmol/L) for 24 hours in the absence or presence of insulin glargine, regular insulin or IGF-1 at various dosages. [Results] The percentages of SubG1 cells in palmitic acid-treated control group, glargine treated group, and regular insulin-treated group were 42.10%±4.24%, 32.00%±3.08%, and 35.97%±3.14%, respectively. The anti-apoptotic effect of insulin glargine was dose-dependent and stronger than regular insulin at all corresponding dosage. [Conclusion] Insulin glargine can dose-dependently protect apoptosis induced by free fatty acid in pancreaticβcells.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2005年第4期413-416,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省社会发展重点攻关项目(2002C30503)
