摘要
目的:建立舒胆通颗粒的质量标准。方法:采用薄层色谱法对舒胆通颗粒中栀子苷、大黄酸和延胡索乙素进行定性鉴别,并采用HPLC测定栀子苷含量。色谱柱:Agilent ZORBAX C18柱(4.6 mm×150 mm,5μm);流动相乙腈-水(15∶85),检测波长238 nm,柱温30℃,流速1 mL.min-1,进样量10μL。结果:在薄层鉴别中能检出栀子苷、大黄酸和延胡索乙素,栀子苷在15.1～151.0 mg.L-1具有良好的线性关系(r=0.999 9);平均回收率为100.45%,RSD 0.48%。结论:使用薄层色谱法对舒胆通颗粒中栀子苷、大黄酸和延胡索乙素进行定性鉴别,操作简便,重现性好,且阴性对照无干扰;栀子苷的含量测定方法简便、快速、准确,具有良好的重复性和回收率,可作为舒胆通颗粒中栀子苷的质量控制方法。
Objective： To establish the quality standard of Shudantong Granules. Method： The jasminoidin, rhein and tetrahydropalmatine in Shudantong Granules were identified by TLC, and the content of jasminoidin was detected by HPLC. The separation was carried out on an Agilent ZORBAX C18column （4.6 mm×250 mm, 5 μm）. The mobile phase consisted of acetonitrile-water （15： 85）. The detective wavelength was set at 238 nm. The column temperature was kept at 30℃. The flow rate was 1.0 mL·min-1 and the injeetion volume was 10μL. Result： The jasminoidin, rhein and tetrahydropalmatine could be identified by TLC and the calibration eurve of jasminoidin showed a good linearity over the range of 15.1-151.0 mg .L-1 （r = 0. 999 9）. The average recovery was 100.45% with RSD 0.48%. Conclusion： This method to identify three main ingredients in Shudantong Granules by TLC is simple and has a great repeatability. The negative control sample has no interference. The method to determine the content of jasminoidin in Shudantong Granules is rapid, accurate and its results have a good repeatability and recovery. This method can be used as the quality control method for this preparation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第7期89-92,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
浙江省中医药管理局项目(2006C021)
