摘要
目的:建立高效液相色谱法测定蓼科植物习见蓼中槲皮素含量的方法。方法:采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,4μm);流动相为甲醇-水-冰醋酸(55:45:1.8),检测波长为370 nm,柱温为35℃,流速为1.0 ml·min^(-1)。结果:槲皮素在0.028～2.210μg范围内线性关系良好,平均回收率为102.4%,RSD=1.04%(n=6)。结论:本法简单、准确,重复性好,可用于习见蓼药材的质量控制。
Objective：To establish a determination method for quercetin in Polygonum plebeium R.Br.by HPLC.Method：The determination was carried out on a Diamonsil C_（18） column（250 mm x4.6 mm,4μm） with methanol-water-acetic acid solution（55：45 ：1.8）as the mobile phase,the detection wavelength was at 370nm,the column temperature was 35℃,and the flow rate was 1.0 ml·min^（-1）.Result：A good linear relationship was shown within the range of 0.028-2.210μg,and the average recovery was 102.4% （RSD -1.04%,n = 6）.Conclusion：The method is simple and reliable with good repeatability,and can be used to control the quality of the medicinal materials.
出处
《中国药师》
CAS
2013年第3期384-386,共3页
China Pharmacist
关键词
高效液相色谱法
习见蓼
槲皮素
含量测定
HPLC
Polygonum plebeium R.Br.
Quercetin
Content determination
