摘要
目的:探讨超微四神丸治疗泄泻的免疫学机制。方法:40只SD大鼠随机分组,制备脾肾阳虚泄泻模型,各组给予不同处理后,采用酶联免疫吸附测定法(ELISA)检测血清中TNF-α、IL-2及IL-4的含量变化。结果:模型组血清TNF-α含量升高,IL-2和IL-4含量降低,与正常组比较差异有统计学意义(P<0.05或P<0.01);四神丸汤剂组和超微组上述指标均有改善,与模型组比较有统计学差异(P<0.05或P<0.01),且超微组IL-2、IL-4的降低程度优于汤剂组(P<0.05)。结论:超微四神丸对泄泻大鼠的作用可能与调节细胞因子的水平有关。
Objective： To investigate the immunological mechanism of Ultrafine Sishen pill on diarrhea.Methods： 40 SD rats were divided into different groups randomly.Prepare spleen-kidney Yang deficiency of diarrhea model.Each group was given different treatments,using enzyme-linked immunosorbent assay（ELISA）to detect the serum levels of TNF-α,IL-2 and IL-4.Results：the content of serum TNF-α in model group increased,contents of IL-2 and IL-4 decreased,the difference was significant compared with normal group（P0.05 or P0.01）.The above-mentioned indexes of Sishen pill decoction group and Ultrafine group were improved,compared with model group they had a significant difference（P0.05 or P0.01）,and ultrafine group in the reduction of IL-2 and IL-4 was superior to the decoction group.Conclusion： Effects of Ultrafine Sishen pill on diarrhea syndrome in rats are probably related to regulation of cytokine levels.
出处
《中医药学报》
CAS
2013年第1期80-81,共2页
Acta Chinese Medicine and Pharmacology
基金
湖南省教育厅科研项目(No.11C0960)
