摘要
目的 :观察脾虚大鼠胃粘膜胃泌素受体的变化规律与益气健脾中药黄芪的调控作用。方法 :应用受体结合法检测胃泌素受体结合位点数。结果 :大黄和利血平脾虚模型大鼠胃粘膜胃泌素受体结合位点数均有明显减少。黄芪在纠正脾虚证的部分病理证候的同时 ,对脾虚大鼠胃粘膜胃泌素受体结合位点数有显著上调作用 ,而对血清胃泌素水平却有一定降低作用趋势。高浓度黄芪注射液在体外可显著促进胃泌素受体结合。结论 :胃泌素受体参与了脾虚证的病理过程 ,黄芪健脾药理可能不是直接作用于胃泌素受体的内源性配基的结合部位 ,而有可能是一种类似酶别构效应机制的 型受体激动剂。
Objective:To observe expression of Gastrin Receptors of Spleen Insufficiency in rats and regulation of Traditional Chinese Medicines.Methods:The number of gastrin receptors of gastric mucosal membrane preparations in rat were determined with radioligand binding assay.Results:The gastrin binding sites of Spleen insufficiency induced by reserpinum or Redix et Rhizoma Rhei markedly dropped in rat gastric mucosal.After Injectio Astragali seu Hedysari,an important traditional Chinese medicine for invigorating Spleen and Qi,the gastrin binding sites increased in rat gastric mucosal while the serum gastrin values decreased.It was further found that Injectio Astragali seu hedysari promoted the gastrin binding at 0.75 and 1.00 mg/ml in vitro.Conclusion:The syndrome of Spleen insufficiency might be a disease related to the abnormal expression of gastrin receptor and perhaps be cured by receptor regulation.
出处
《中国中医基础医学杂志》
CAS
CSCD
2000年第5期36-40,共5页
JOURNAL OF BASIC CHINESE MEDICINE
