摘要
为研究不同人群载脂蛋白 (Apo)E基因多态性的分布特征 ,采用聚合酶链 -限制性片段长度多态性 (PCR -RFLP)方法 ,先扩增出ApoE基因第 4外显子内的 2 2 7bp片段 ,继以限制性内切酶CfoI酶解消化 ,8%聚丙烯酰胺凝胶电泳 ,最后银染显带判定基因型。结果正常汉族、朝族、回族和蒙古族ApoE等基因频率 ,ε2 等位基因频率分别是 8 0 %、12 9%、11 3%和 12 6% ,ε3分别是 85 7%、78 6%、79 1%和 79 7% ,ε4 分别是 6 3%、8 6%、9 7%和 7 9%。说明汉族、朝族、回族和蒙古族ApoE等位基因频率与亚洲的日本和新加坡人群结果相近 。
In this paper,the distribution of Apolipoprotein E polymorphism in different populations was studied.The polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technique was used to amplify 227 base-pair DNA fragments in the fourth exon of ApoE gene and to digest them with restriction endonuclease enzyme CfoI.Then electrophoresis on 8% polyacrylamide gel to identify the products.Their genotypes were determined subsequently by silver staining,and allele frequencies were calculated.Results indicated that in healthy individuals of Han,Chao,Hui and Mongolian ethnical group,the allelic frequencies of AopE,ε 2 were 8 0%,12 9%,11 3% and 12 6% respectively,ε 3 were 85 7%,78 6%,79 1% and 79 7% respectively,ε 4 was 6 3%,8 6%,9 7% and 7 9% respectively.We concluded that the distribution of apolipoprotein E gene polymorphism in the Han,Chao,Hui and Mongolian nationalities is similar to that in Asian Japan and Singapore population,but it is largely different from those of Europe and America populations.
出处
《中国医师杂志》
CAS
2000年第9期530-531,534,共3页
Journal of Chinese Physician
